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| Experimental setup. © Z. Benderlioglu CC BY 4.0 |
visual information alone, according to new research from The Ohio State University. The findings suggest that horses are capable of rapidly assessing danger without relying on sound, scent or previous experience, while also revealing that they may conceal signs of stress despite experiencing significant physiological arousal.
As prey animals, horses have evolved sophisticated mechanisms for detecting threats. Previous research has shown that they respond to predators through increased vigilance, flight behaviour and elevated stress levels. However, most studies have examined combinations of visual, auditory and olfactory cues. The latest study is among the first to isolate vision alone, demonstrating that horses can identify a predator even when it is presented as a silent video image.
The researchers studied 18 horses of varying ages and both
sexes housed at The Ohio State
University Equine Center. Each horse watched a
series of 20-second silent video clips projected onto a screen while wearing a
heart rate monitor and being filmed for behavioural analysis.
The control footage showed grazing wombats, a species unfamiliar to the horses but posing no threat. This was followed by clips of wolves, also unfamiliar to the horses, displaying either affiliative behaviours such as grooming or aggressive interactions.
Despite the absence of sound, movement towards the horses or any scent cues, the animals reacted very differently to the two species. Heart rates remained close to baseline while the horses watched the wombats, but increased significantly whenever wolves appeared on the screen. Surprisingly, there was no difference in the horses' responses to wolves that were fighting compared with those calmly grooming one another, suggesting that the horses identified the animals themselves as potential predators rather than responding to the behaviour they were displaying.
Lead author Zeynep Benderlioglu explained that the findings indicate horses engage in rapid cognitive assessment rather than immediately entering a classic fight-or-flight response. Although their heart rates increased markedly, they showed few obvious behavioural signs of anxiety.
Unlike a startled horse that might snort, toss its head or attempt to flee, these horses largely maintained a calm outward appearance while internally experiencing heightened alertness. This disconnect has important implications for both equine welfare and rider safety, as handlers may assume a horse is relaxed when it is actually processing a potential threat.
The study also found differences between individual horses. Male horses exhibited stronger heart rate responses to the wolf videos than females, despite having similar resting heart rates. Horses occupying higher social positions within the herd also showed greater physiological responses. The researchers suggest that dominant horses may play a key role in monitoring danger because other members of the herd often rely on them when making collective decisions about safety.
Age also influenced behaviour, with older horses showing lower levels of fearfulness and social dependency than younger animals.
The team also investigated how the horses viewed the videos. Previous research has suggested horses often use their left eye when assessing threats because the right side of the brain is specialised for processing danger. However, the horses in this study showed almost no preference for left-eye viewing while watching the wolves. Instead, they displayed no clear lateralised gaze, while the harmless wombats were viewed predominantly using binocular vision, suggesting exploratory rather than defensive processing.
The researchers believe this unexpected finding highlights the complexity of equine cognition. Rather than reacting automatically, the horses appeared to evaluate the visual information before deciding that no immediate action was necessary.
Overall, the study demonstrates that horses possess a sophisticated ability to recognise unfamiliar predators using vision alone while masking their internal state. The findings provide new insight into how horses perceive potential threats and underline the importance of recognising that a horse may be experiencing significant stress even when it appears outwardly calm.
For more details, see:
Hofacker R, Sebunia N, Pihlblad J, Benderlioglu Z (2026)
Recognition of unfamiliar predators in domestic horses through only visual predator cues. PLoS One 21(7): e0349298.
https://doi.org/10.1371/journal.pone.0349298





