Wednesday, April 24, 2019

Mild asthma can separate winners from losers on the racetrack

Dr Laurent Couetil treats asthmatic horse with a nebuliser


A split second may be all that separates the winner and the rest of the field. Anything that limits peak performance reduces the chance of success. In many cases the problem lies in the lungs. 

A recent study has shown that mild equine asthma is common in racing horses and negatively impacts performance.

The study, led by Laurent Couëtil, director of Purdue University’s Equine Sports Medicine Center, found that approximately 80% of racehorses had evidence of low-grade inflammation in their lungs and that the greater the lung inflammation, the poorer horses performed. 

Couëtil explains: “Unlike the heart or muscle, the lung in the horse athlete is a limiting factor. Even in healthy horses, breathing is a limiting factor on performance. So, if you take a little bit away from that, the consequences can be severe.”

Thoroughbred horses, trained in eight separate yards, were recruited to the study, which was carried out at the Shelbyville, Indiana, racetrack. 

Horses were examined an hour after the race. The examination included endoscopy of the respiratory tract, and the collection of a sample of cells from deep within the lungs (bronchoalveolar lavage - BAL)

Then, within a week after the race, the research team measured the amount of dust in the breathing zone of the horses. Horses were fitted with sensors near their noses to measure how much dust they were inhaling. They wore the sensors while going about their daily business, and the findings show that most of the dust they inhaled was coming from hay.

The research team found that the greater the exposure to dust, the worse the lung inflammation. However, it was not just any type of dust, but “respirable dust” (particles that are less than 4 microns in diameter - too small to be seen with the naked eye, and so small that they can penetrate deep into the lungs) that was most important.

“We know dust is the problem, but now we’re trying to figure out how to reduce it. In our next study, we’re testing different types of hay to see if we can reduce the amount of dust horses are coming into contact with while they’re eating,” Couëtil said.

Some horses will be fed steamed hay, which incubates in a sauna-like case for an hour before being fed to horses. The final product is a little wet, but the process kills much of the mould and dust that accumulates in bales of hay. Other horses will be fed baled silage, or haylage, which is hay baled at a higher moisture content than dry hay and stored in a tightly sealed plastic wrap.

Previous work* by Couëtil and colleagues found that some supplements could also help horses recover from severe asthma. When fed together with a lower-dust feed option, Omega-3 fatty acid supplements were shown to enhance and hasten recovery.

“The horses that were fed the supplement improved much quicker and to a much greater extent. Many of them stopping coughing within a couple weeks,” Couëtil said. “The next step for us is trying to understand the mechanism that makes that happen.”

For more details, see:

An observational study of environmental exposures, airway cytology, and performance in racing thoroughbreds.
Kathleen M. Ivester, Laurent L. Couëtil, George E. Moore.
Journal of Veterinary Internal Medicine (2018) Vol 32, pp 1754-1762

*Omega‐3 Fatty Acid Supplementation Provides an Additional Benefit to a Low‐Dust Diet in the Management of Horses with Chronic Lower Airway Inflammatory Disease
N. Nogradi,  L.L. Couetil, J. Messick,  M.A. Stochelski, J.R. Burgess
J Vet Intern Med. (2015) 29(1): 299–306.
 


Tuesday, April 23, 2019

Permafrost gives up 40,000 year-old foal


Global warming is revealing secrets that have been hidden in the permafrost for many thousands of years. In August 2018, researchers found the frozen carcass of a foal of a now extinct species of horse in the Yakutsk region of north eastern Russia.

https://www.s-vfu.ru/en/news/detail.php?SECTION_ID=&ELEMENT_ID=115956
Believed to be only a few weeks old when it died, the colt foal is so well preserved that even its mane and tail are intact. It is thought that it drowned in soft mud that then became part of the permafrost.

The foal is thought to have lived over 40,000 years ago in the Upper Paleolithic (Late Stone Age), and was found in the Batagaika crater in Yakutia, the coldest region in Russia.

The North-Eastern Federal University (NEFA) in Yakutsk recently hosted a paleontological seminar, attended by NEFA Scientists as well as specialists from the Yakutsk Research Institute of Agriculture, and the Korean Sooam Biotech Research Foundation to discuss the study of the ancient foal. 

Computed tomography of the foal carcass was used to examine the internal structure and create a 3D model of the body. Sergey Vasilev, head of the Center for 3-D Modelling and Virtual Reality of NEFU Institute of Physics and Technologies, noted that this allows them to create a complete picture of what the foal looked like. 

Semyon Grigoriev, head of P.A. Lazarev Mammoth Museum of NEFU Institute of Applied Ecology of the North, added “In case of finding the remains of unique discoveries, we always try to organize their computed tomography, because with its help we can fix the current preservation after the moment of discovery without disturbing the integrity of tissues or organs”.

According to Lena Grigorieva, lead researcher at the International Center for Collective Use of Molecular Paleontology, the foal is unique in its state of preservation. Materials collected have been submitted for microbiological, histological and cellular studies. 

“The study of materials will give a general idea of ​​the microflora of the ancient horse”, said Yan Ahremenko, an associate professor of the Department of Histology and Microbiology at NEFU Medical Institute. “Perhaps we will find lactic acid microorganisms and ancient bifidobacteria as in the case of the mammoth.”

The Siberian Times quotes Dr Semyon Grigoryev: “The autopsy shows beautifully preserved internal organs. Samples of liquid blood were taken from heart vessels… The muscle tissues preserved their natural reddish colour,”  

The scientists hope to be able to find viable genetic material that could be used to attempt to clone the foal. 

South Korean cloning expert Professor Hwang Woo Suk said, “If we manage to find a cell, then we will do our best to clone the unique animal.’ 

For more details, see:

NEFU website:



Monday, April 22, 2019

Diagnosing equine asthma


 “Equine asthma” is now recognised as a diagnosis that encompasses both inflammatory airway disease (with less severe signs, virtually unnoticeable when the horse is at rest) and recurrent airway obstruction or heaves (with increased breathing efforts at rest and chronic coughing).

Dr Laurent Couëtil and his colleagues at the Purdue University College of Veterinary Medicine have developed a new technique to overcome the challenge of testing equine pulmonary function.

“Milder equine asthma has been difficult to detect because horses don’t necessarily show many signs besides the fact that they’re not performing well. Some of them cough once in a while, but it isn’t crippling them,” Couëtil said. “Now that we have the tools to look for it, we realize it’s very common.”
In a study 80% racehorses had signs of asthma ((c) Purdue university/Rebecca Wilcox)

To test for equine asthma, veterinarians use variations of methods developed to measure lung disease and dysfunction in humans, such as the bronchoalveolar lavage and lung function test. However, some of these tests are not commonly done or are impossible to perform on horses. 

 “In humans, the most common test performed to test for asthma is forced exhalation. The nurse trains you to take in the deepest breath possible and blow out as hard as you can,” he said. “This is easy for people because we can follow instructions, but you can’t tell a horse to do that, so I worked with Purdue engineers to develop a pulmonary function test for horses.”

The test uses a mechanical ventilator to control a sedated horse’s breathing. A series of tanks, using positive and negative pressure, help mimic deep inhalation and exhalation. During the process, a computer records data about the patient’s lung capacity, expiration volume and expiration flow. This system is the only one in the world capable of performing such a test, and is extremely sensitive, detecting even very mild asthma. The equine pulmonary function test technology is patented through Purdue’s Office of Technology Commercialization.

Currently, the equipment is too large to be used other than in the hospital.  It also requires specialised expertise to operate it. The lab is working to develop a more simplified and portable system that could be used by ambulatory veterinarians to diagnose asthma in the field.

Read more: