Friday, July 04, 2014

Eclipse clone on the way?

News has emerged that scientists have successfully grown cells containing the DNA of Eclipse, the most famous racehorse of all time. This is the first, and most difficult step to producing a live cloned foal.

Eclipse, born two hundred and fifty years ago, is widely considered to be the greatest Thoroughbred racehorse ever. Unbeaten throughout his racing career from 1769-1770, Eclipse  was in different league to his contemporaries and won eight of his races by default –  “walk overs” - as no-one would put up a horse to run against him.

After retiring from racing he became a very successful sire. It is likely that there are very few Thoroughbred horses alive today that do not contain a link to Eclipse somewhere in their bloodlines.

His skeleton currently adorns the teaching block named after him at the Royal Veterinary College, London.

Attempts to recover DNA from his much studied skeleton proved fruitless, but samples of his tail hair which had been woven into the tassel of "The Whip”, the prize of victory in the self-named race, were found to contain sufficient undamaged genetic material for the scientists to work with.

A team from the University of London’s Department of Veterinary Embryology have been able to transfer DNA from Eclipse into the cytoplasm of cells from the subcutaneous connective tissue of a donor horse. These fibroblasts have then been proliferated in the lab and have been shown to have remained viable and to contain the replicated Eclipse DNA.

The next stage in the cloning process, which is due to start this month, will be the implantation of the chromosomal tissue into an egg from a donor mare and then the chemical signalling to trigger the egg to start the development into an embryo. After the recombined oocyte has been activated, it is transferred surgically to the oviduct of a recipient mare, which carries the foal to birth. If all goes according to plan, a cloned Eclipse foal could be born in early 2015.

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